@article {70,
	title = {The geographical segregation of human lice preceded that of Pediculus humanus capitis and Pediculus humanus humanus},
	journal = {Comptes Rendus Biologies},
	volume = {326},
	year = {2003},
	note = {LR: 20041117; JID: 101140040; 0 (DNA Primers); 0 (Peptide Elongation Factor 1); 0 (RNA, Ribosomal, 18S); ppublish},
	month = {2003},
	pages = {565 - 574},
	address = {France},
	abstract = {In order to investigate human-louse phylogeny, we partially sequenced two nuclear (18S rRNA and EF-1 alpha) and one mitochondrial (COI) genes from 155 Pediculus from different geographical origins. The phylogenetic analysis of 18S rRNA and EF-1 alpha sequences showed that human lice were classified into lice from Sub-Saharan Africa and lice from other areas. In both clusters, head and body lice were clearly grouped into two separate clusters. Our results indicate that the earliest divergence within human pediculidae occurred between African lice and other lice, and the divergence between head and body lice was not the result from a single event.},
	keywords = {animals, Base Sequence, dna, geography, humans, Pediculus, phylogeny, polymerase, RNA, Ribosomal, 18S/genetics},
	isbn = {1631-0691},
	url = {http://phthiraptera.info/sites/phthiraptera.info/files/45968.pdf},
	author = {Yong,Z. and Fournier,P. E. and Rydkina,E. and Raoult,Didier}
}
@article {75,
	title = {Molecular evolution of Pediculus humanus and the origin of clothing},
	journal = {Current Biology},
	volume = {13},
	year = {2003},
	note = {LR: 20050127; GENBANK/AY316748; GENBANK/AY316749; GENBANK/AY316750; GENBANK/AY316751; GENBANK/AY316752; GENBANK/AY316753; GENBANK/AY316754; GENBANK/AY316755; GENBANK/AY316756; GENBANK/AY316757; GENBANK/AY316758; GENBANK/AY316759; GENBANK/AY316760; GENBANK/AY316761; GENBANK/AY316762; GENBANK/AY316763; GENBANK/AY316764; GENBANK/AY316765; GENBANK/AY316766; GENBANK/AY316767; GENBANK/AY316768; GENBANK/AY316769; GENBANK/AY316770; GENBANK/AY316771; GENBANK/AY316772; GENBANK/AY316773; GENBANK/AY316774; GENBANK/AY316775; GENBANK/AY316776; GENBANK/AY316777; GENBANK/AY316778; GENBANK/AY316779; GENBANK/AY316780; GENBANK/AY316781; GENBANK/AY316782; GENBANK/AY316783; GENBANK/AY316784; GENBANK/AY316785; GENBANK/AY316786; GENBANK/AY316787; GENBANK/AY316788; GENBANK/AY316789; GENBANK/AY316790; GENBANK/AY316791; GENBANK/AY316792; GENBANK/AY316793; GENBANK/AY316794; GENBANK/AY316795; GENBANK/AY316796; GENBANK/AY316797; GENBANK/AY316798; GENBANK/AY316799; GENBANK/AY316800; GENBANK/AY316801; GENBANK/AY316802; GENBANK/AY316803; GENBANK/AY316804; GENBANK/AY316805; GENBANK/AY316806; GENBANK/AY316807; GENBANK/AY316808; GENBANK/AY316809; GENBANK/AY316810; GENBANK/AY316811; GENBANK/AY316812; GENBANK/AY316813; GENBANK/AY316814; GENBANK/AY316815; GENBANK/AY316816; GENBANK/AY316817; GENBANK/AY316818; GENBANK/AY316819; GENBANK/AY316820; GENBANK/AY316821; GENBANK/AY316822; GENBANK/AY316823; GENBANK/AY316824; GENBANK/AY316825; GENBANK/AY316826; GENBANK/AY316827; GENBANK/AY316828; GENBANK/AY316829; GENBANK/AY316830; GENBANK/AY316831; GENBANK/AY316832; GENBANK/AY316833; GENBANK/AY316834; GENBANK/AY316835; GENBANK/AY316836; GENBANK/AY316837; GENBANK/AY316838; GENBANK/AY316839; GENBANK/AY316840; GENBANK/AY316841; GENBANK/AY316842; GENBANK/AY316843; GENBANK/AY316844; GENBANK/AY316845; GENBANK/AY316846; GENBANK/AY316847; GENBANK/AY316848; GENBANK/AY316849; GENBANK/AY316850; GENBANK/AY316851; GENBANK/AY316852; GENBANK/AY316853; GENBANK/AY316854; GENBANK/AY316855; GENBANK/AY316856; GENBANK/AY316857; GENBANK/AY316858; GENBANK/AY316859; GENBANK/AY316860; GENBANK/AY316861; GENBANK/AY316862; GENBANK/AY316863; GENBANK/AY316864; GENBANK/AY316865; GENBANK/AY316866; GENBANK/AY316867; GENBANK/AY316868; GENBANK/AY316869; GENBANK/AY316870; GENBANK/AY316871; GENBANK/AY316872; GENBANK/AY316873; GENBANK/AY316874; GENBANK/AY316875; GENBANK/AY316876; GENBANK/AY316877; GENBANK/AY316878; GENBANK/AY316879; GENBANK/AY316880; GENBANK/AY316881; GENBANK/AY316882; GENBANK/AY316883; GENBANK/AY316884; GENBANK/AY316885; GENBANK/AY316886; GENBANK/AY316887; GENBANK/AY316888; GENBANK/AY316889; GENBANK/AY316890; GENBANK/AY316891; GENBANK/AY316892; GENBANK/AY316893; GENBANK/AY316894; GENBANK/AY316895; GENBANK/AY316896; GENBANK/AY316897; GENBANK/AY316898; GENBANK/AY316899; GENBANK/AY316900; GENBANK/AY316901; GENBANK/AY316902; GENBANK/AY316903; GENBANK/AY316904; GENBANK/AY316905; GENBANK/AY316906; GENBANK/AY316907; GENBANK/AY316908; GENBANK/AY316909; GENBANK/AY316910; GENBANK/AY316911; GENBANK/AY316912; GENBANK/AY316913; GENBANK/AY316914; GENBANK/AY316915; GENBANK/AY316916; GENBANK/AY316917; GENBANK/AY316918; GENBANK/AY316919; GENBANK/AY316920; GENBANK/AY316921; GENBANK/AY316922; GENBANK/AY316923; GENBANK/AY316924; GENBANK/AY316925; GENBANK/AY316926; GENBANK/AY316927; GENBANK/AY316928; GENBANK/AY316929; JID: 9107782; 0 (DNA, Mitochondrial); EIN: Curr Biol. 2004 Dec 29;14(24):2309; ppublish},
	month = {2003},
	pages = {1414 - 1417},
	abstract = {The human head louse (Pediculus humanus capitis) and body louse (P. humanus corporis or P. h. humanus) are strict, obligate human ectoparasites that differ mainly in their habitat on the host : the head louse lives and feeds exclusively on the scalp, whereas the body louse feeds on the body but lives in clothing. This ecological differentiation probably arose when humans adopted frequent use of clothing, an important event in human evolution for which there is no direct archaeological evidence. We therefore used a molecular clock approach to date the origin of body lice, assuming that this should correspond with the frequent use of clothing. Sequences were obtained from two mtDNA and two nuclear DNA segments from a global sample of 40 head and body lice, and from a chimpanzee louse to use as an outgroup. The results indicate greater diversity in African than non-African lice, suggesting an African origin of human lice. A molecular clock analysis indicates that body lice originated not more than about 72,000 +/- 42,000 years ago; the mtDNA sequences also indicate a demographic expansion of body lice that correlates with the spread of modern humans out of Africa. These results suggest that clothing was a surprisingly recent innovation in human evolution.},
	keywords = {Africa, animals, Clothing, dna, Evolution, Molecular, history, humans, Lice Infestations/history/parasitology, Molecular Sequence Data, Pediculus, Research Support, Non-U.S. Gov{\textquoteright}t, Time Factors},
	isbn = {0960-9822},
	author = {Kittler,R. and Kayser,M. and Stoneking,M.}
}
@article {81,
	title = {Real-time PCR duplex assay for Rickettsia prowazekii and Borrelia recurrentis},
	journal = {Annals of the New York Academy of Sciences},
	volume = {990},
	year = {2003},
	note = {LR: 20041117; JID: 7506858; 0 (DNA Primers); 0 (DNA, Bacterial); ppublish},
	month = {2003},
	pages = {302 - 310},
	address = {United States},
	abstract = {Rickettsia prowazekii, the etiologic agent for epidemic typhus, and Borrelia recurrentis, the etiologic agent of relapsing fever, both utilize the same vector, the human body louse (Pediculus humanus), to transmit human disease. We have developed an assay to detect both bacterial pathogens in a single tube utilizing real-time PCR. Assays for both agents are specific. The R. prowazekii and B. recurrentis assays do not detect nucleic acid from R. typhi, R. canada, or any of eight spotted fever rickettsiae. In addition they did not react with Neorickettsia risticii, N. sennetsu, Franciscella persica, Bartonella quintana, Legionella pneumophila, Proteus mirabilis, Salmonella enterica, Escherichia coli, and Staphylococcus aureus. Moreover, the B. recurrentis assay did not detect B. duttonii, B. coriaceae, B. afzelii, B. garinii, B. hermsii, or B. burgdorferi nucleic acid. Both assays detected repeatedly only R. prowazekii or B. recurrentis either when tested alone or together in one test tube.},
	keywords = {animals, Base Sequence, Borrelia, Comparative Study, dna, genes, humans, Lice/microbiology, polymerase, Research Support, Non-U.S. Gov{\textquoteright}t, Rickettsia prowazekii/classification/genetics/isolation \& purification},
	isbn = {0077-8923},
	author = {Jiang,J. and Temenak,J. J. and Richards,A. L.}
}
@article {84,
	title = {Detection of Bartonella quintana from body lice (Anoplura: Pediculidae) infesting homeless people in Tokyo by molecular technique},
	journal = {Journal of Medical Entomology},
	volume = {39},
	year = {2002},
	month = {2002},
	pages = {427 - 429},
	abstract = {We report detection of Bartonella quintana Brenner, the pathogenic agent of trench fever, from body lice, Pediculus humanus L., infesting homeless people in Tokyo by polymerase chain reaction. Two of 12 (16.7\%) homeless were infested with Bartonella-positive body lice. From the current status of the recent increase of homeless people in many large cities of the developed countries, a medical examination of homeless people should be carefully performed in the consideration of trench fever. Sampling of body lice from clothing of homeless people is recommended for quick and accurate diagnosis of trench fever through the detection of B. quintana DNA.},
	keywords = {animals, Bartonella quintana/genetics/isolation \& purification, dna, homeless, humans, Japan, Lice Infestations/parasitology, Pediculus, polymerase, Research Support, Non-U.S. Gov{\textquoteright}t, trench fever},
	url = {http://www.phthiraptera.info/Publications/44866.pdf},
	author = {Sasaki,Toshinori and Kobayashi,Mutsuo and Agui,Noriaki}
}
@article {85,
	title = {Intragenomic variation in ITS2 rDNA in the louse of humans, Pediculus humanus: ITS2 is not a suitable marker for population studies in this species},
	journal = {Insect Molecular Biology},
	volume = {11},
	year = {2002},
	month = {2002},
	pages = {651 - 657},
	abstract = {The two internal transcribed spacers (ITS) of ribosomal DNA are often used as markers of populations of insects. We studied the ITS2 of the head lice and body lice of humans, to determine whether this gene is a suitable marker of populations of these insects. ITS2 sequences were amplified by PCR from lice from four different countries: Australia, China, Japan and the USA. Direct cycle-sequencing of some of these PCR products gave equivocal nucleotide chromatograms. This indicated that some lice had more than one ITS2 sequence, so we cloned PCR products from these lice. Temperature gradient gel electrophoresis (TGGE) revealed that 50 of the 67 clones we screened had different nucleotide sequences. All lice had several ITS2 types, including those with unequivocal chromatograms. A phylogenetic tree of 15 different ITS2 sequences showed that the sequences from individual lice were not monophyletic. We conclude that the ITS2 is not a useful marker of populations for Pediculus humanus.},
	keywords = {animals, Australia, Base Sequence, China, dna, geography, humans, Introns, Japan, Lice Infestations/parasitology, Molecular Sequence Data, Pediculus, phylogeny, polymerase, Research Support, Non-U.S. Gov{\textquoteright}t, sequence, U.S.A., variation},
	url = {http://www.phthiraptera.info/Publications/44841.pdf},
	author = {Leo,Natalie P. and Barker,Stephen C.}
}
@article {56,
	title = {Cloning and characterization of a trypsin-encoding cDNA of the human body louse Pediculus humanus},
	journal = {Insect Molecular Biology},
	volume = {13},
	year = {2004},
	note = {LR: 20041117; JID: 9303579; 0 (DNA Primers); EC 3.4.21.4 (Trypsin); ppublish},
	month = {2004},
	pages = {9 - 18},
	address = {England},
	abstract = {From a cDNA library of the whole insect, a trypsin gene of Pediculus humanus has been cloned and sequenced. The 908 bp clone has an open reading frame of 759 bp, which encodes a pre-proenzyme with 253 amino acid residues. A sixteen-residue N-terminal signal peptide is followed by a twelve-residue activation peptide with putative cleavage sites at Gly16 and Tyr28. The deduced amino acid sequence has several features typical of trypsin proteases and an overall identity of 35-43\% with the trypsins of several haematophagous Diptera. The 1.0 kb genomic trypsin gene contains three introns of 102, 79 and 80 nucleotides following the codons for Gly16, Gln74 and Ala155, respectively. Only a single gene seems to be present. In Northern blot analysis, unfed first instar larvae have an identical or slightly lower level of trypsin mRNA than fed adult lice, and in adults 2-24 h after the bloodmeal this gene shows a constitutive expression. After in vitro transcription and translation, the activation peptide is cleaved by chymotrypsin, a so far unreported phenomenon in trypsin activation.},
	keywords = {Amino Acids, animals, Base Sequence, Blotting, Northern, Comparative Study, dna, electrophoresis, genes, Molecular Sequence Data, Pediculus, polymerase, Research Support, Non-U.S. Gov{\textquoteright}t, sequence, Transition Temperature, Trypsin/genetics},
	isbn = {0962-1075},
	author = {Kollien,A. H. and Waniek,P. J. and Pr{\"o}ls,F. and Habedank,Birgit and Schaub,G. A.}
}
