@article {68,
	title = {An automated feeding apparatus for in vitro maintenance of the human head louse, Pediculus capitis (Anoplura: Pediculidae)},
	journal = {Journal of Medical Entomology},
	volume = {40},
	year = {2003},
	month = {2003},
	pages = {795 - 799},
	abstract = {An automated feeding apparatus was developed to maintain the human head louse (Pediculus capitis DeGeer) in vitro. With the use of valves and timers, banked human blood and saline from refrigerated reservoirs were pumped into and flushed out of the system every 7 d. During this rotational interval, bloodmeals were provided to head lice continuously and ad libitum through a stretched Nescofilm-silicone sandwich membrane. Compared with our previous in vitro human head louse-rearing apparatus, greater numbers of lice could be fed simultaneously with minimal human monitoring. Development of second to third instars and third instars to adults was significantly faster when lice were reared in vivo than on either of the in vitro rearing systems; there was no significant difference in the duration of the first instar. Although fecundity and hatch rates were significantly higher for female lice reared in vivo, similar trends have been observed for other membrane-fed arthropods. Body lice (Pediculus humanus L.) and bed bugs (Cimex lectularius [L.]) also completed most of their life cycle on this apparatus. Our automated mass-rearing system has broad applications for maintaining fluid-sucking ectoparasites and will facilitate various toxicological, behavioral, and disease-transmission investigations.},
	keywords = {animals, Animals, Laboratory, Automation, Feeding Behavior, fertility, humans, larva, Lice Infestations/parasitology, Life Cycle Stages, Longevity, Pediculus, Research Support, U.S. Gov{\textquoteright}t, P.H.S.},
	url = {http://www.phthiraptera.info/Publications/44749.pdf},
	author = {Takano-Lee,Miwa and Velten,R. K. and Edman,John D. and Mullens,B. A. and Clark,J. Marshall}
}
@article {71,
	title = {In vivo and in vitro rearing of Pediculus humanus capitis (Anoplura: Pediculidae)},
	journal = {Journal of Medical Entomology},
	volume = {40},
	year = {2003},
	month = {2003},
	pages = {628 - 635},
	abstract = {Four geographically distinct colonies of the human head louse, Pediculus humanus capitis De Geer (Anoplura: Pediculidae) were reared on a live host and exhibited significantly different life history patterns. Florida head lice exhibited approximately 10\% slower development and approximately 15\% reduced longevity relative to California or Ecuador head lice. Fecundity (4.9 +/- 0.2 eggs/female/d) and fertility (76.4 +/- 2.9\% mean hatching rate) declined over the lifetime of female lice, especially when separated from males (i.e., unmated recently). All four colonies (above plus one from Panama) were similar in their ability to tolerate starvation, although older stages tended to die sooner. An in vitro feeding apparatus was developed to rear head lice. Teneral first instar lice were placed on human hair tufts on the upper side of membrane-covered feeders, which were immersed bottom-side down within a vessel containing warmed human blood. Relative to lice reared on a human host, in vitro-reared lice required a significantly longer time (10-20\%) to molt and survived a significantly shorter time as adults (30-50\%); the addition of antibiotics did not adversely affect louse development. Teneral first instars were more likely than any other stage to feed through the membrane. Lice spent a significantly greater proportion of time searching in the in vitro apparatus than on a host, but the proportion of time spent feeding did not differ. This research is the first to demonstrate that head lice can be reared successfully in vitro through a complete life cycle.},
	keywords = {animals, Anti-Bacterial Agents/pharmacology, Feeding Behavior, geography, humans, Lice Infestations/parasitology/physiopathology, Molting/physiology, Pediculus, Research Support, U.S. Gov{\textquoteright}t, P.H.S.},
	url = {http://www.phthiraptera.info/Publications/44763.pdf},
	author = {Takano-Lee,Miwa and Yoon,Kyong Sup and Edman,John D. and Mullens,B. A. and Clark,J. Marshall}
}
@article {61,
	title = {Home remedies to control head lice: assessment of home remedies to control the human head louse, Pediculus humanus capitis (Anoplura: Pediculidae)},
	journal = {Journal of Pediatric Nursing},
	volume = {19},
	year = {2004},
	note = {JID: 8607529; 0 (Plant Oils); 64-19-7 (Acetic Acid); 67-63-0 (2-Propanol); 7732-18-5 (Water); 8001-25-0 (olive oil); 8009-03-8 (Petrolatum); 8029-34-3 (Butter); ppublish},
	month = {2004},
	pages = {393 - 398},
	abstract = {As the frequency and level of pediculicide resistance increases throughout the world, the need for novel solutions to control pediculosis has intensified. The development and registration of new pesticides has become so costly that many chemical companies are unwilling to pursue it and health-care providers now face a serious lack of new commercial pediculicides. Many infested people resort to using "home-remedy" approaches that have not been scientifically tested. In this article, we examined the potential value of six purportedly effective "home remedies" (vinegar, isopropyl alcohol, olive oil, mayonnaise, melted butter, and petroleum jelly) to treat head louse infestations and the likelihood of drowning lice by water submersion. Results indicated that only the application of petroleum jelly caused significant louse mortality but no treatment prevented lice from laying eggs. Most home remedy products did little to kill eggs, despite prolonged exposure. Petroleum jelly caused the greatest egg mortality, allowing only 6\% to hatch. It was extremely difficult to drown lice, despite extended periods (i.e., 8 hr) of water submersion, suggesting that killing lice by depriving them of oxygen is inefficient. None of the home remedy products we surveyed was an effective means of louse control. This suggests that when treatment failure occurs, an increased amount of time and effort should be focused on alternative chemical pediculicides and/or manual louse removal (i.e., combing) rather than using any of these products.},
	keywords = {Acetic Acid, animals, Butter, egg, humans, lice, Ovum, Pediculus, Pediculus humanus capitis, Plant Extracts, Research Support, U.S. Gov{\textquoteright}t, P.H.S., Self Care},
	isbn = {0882-5963},
	author = {Takano-Lee,Miwa and Edman,John D. and Mullens,B. A. and Clark,J. Marshall}
}
@article {62,
	title = {Insecticidal activity of plant essential oils against Pediculus humanus capitis (Anoplura: Pediculidae)},
	journal = {Journal of Medical Entomology},
	volume = {41},
	year = {2004},
	month = {2004},
	pages = {699 - 704},
	abstract = {The insecticidal activity of 54 plant essential oils against female Pediculus humanus capitis De Geer was examined using direct contact and fumigation methods, and compared with the lethal activity of delta-phenothrin and pyrethrum, two commonly used pediculicides. In a filter paper contact bioassay with female P. humanus capitis, the pediculicidal activity was more pronounced in eucalyptus, marjoram, pennyroyal, and rosemary oils than in delta-phenothrin and pyrethrum on the basis of LT50 values at 0.0625 mg/cm2. At 0.125 mg/cm2, pediculicidal activity of cade, cardamone ceylon, clove bud, myrtle, rosewood, and sage oils was comparable with that of the test insecticides. In fumigation tests with female P. humanus capitis at 0.25 mg/cm2, eucalyptus, marjoram, pennyroyal, and rosemary oils were more effective in closed containers than in open ones, indicating that the effect of these oils was largely a result of action in the vapor phase. Neither delta-phenothrin nor pyrethrum exhibited fumigant toxicity. The essential oils described herein merit further study as potential control agents for P. humanus capitis.},
	keywords = {animals, Fumigation/methods, insecticide, Oils, Volatile/toxicity, Pediculus, Plant Extracts, Research Support, Non-U.S. Gov{\textquoteright}t, Species Specificity},
	url = {http://www.phthiraptera.info/Publications/44706.pdf},
	author = {Yang,Young-Cheol and Lee,Hoi-Seon and Clark,J. Marshall and Ahn,Young-Joon}
}
@article {64,
	title = {Ovicidal and adulticidal activity of Eucalyptus globulus leaf oil terpenoids against Pediculus humanus capitis (Anoplura: Pediculidae)},
	journal = {Journal of Agricultural and Food Chemistry},
	volume = {52},
	year = {2004},
	note = {LR: 20041117; JID: 0374755; 0 (Plant Oils); 0 (Terpenes); ppublish},
	month = {2004},
	pages = {2507 - 2511},
	address = {United States},
	abstract = {The toxic effects of Eucalyptus globulus leaf oil-derived monoterpenoids [1,8-cineole, l-phellandrene, (-)-alpha-pinene, 2-beta-pinene, trans-pinocarveol, gamma-terpinene, and 1-alpha-terpineol] and the known Eucalyptusleaf oil terpenoids (beta-eudesmol and geranyl acetate) on eggs and females of the human head louse, Pediculus humanus capitis, were examined using direct contact and fumigation bioassays and compared with the lethal activity of delta-phenothrin and pyrethrum, two commonly used pediculicides. In a filter paper contact bioassay with female P. h. capitis, the pediculicidal activity was more pronounced with Eucalyptus leaf oil than with either delta-phenothrin or pyrethrum on the basis of LT(50) values (0.125 vs 0.25 mg/cm(2)). 1,8-Cineole was 2.2- and 2.3-fold more toxic than either delta-phenothrin or pyrethrum, respectively. The pediculicidal activities of (-)-alpha-pinene, 2-beta-pinene, and (E)-pinocarveol were comparable to those of delta-phenothrin and pyrethrum. l-Phellandrene, gamma-terpinene, and 1-alpha-terpineol were relatively less active than delta-phenothrin and pyrethrum. beta-Eudesmol and geranyl acetate were ineffective. 1-alpha-Terpineol and (E)-pinocaveol were highly effective at 0.5 and 1.0 mg/cm(2), respectively, against P. h. capitis eggs. At 1.0 mg/cm(2), (-)-alpha-pinene, 2-beta-pinene, and gamma-terpinene exhibited moderate ovicidal activity, whereas little or no ovicidal activity was observed with the other terpenoids and with delta-phenothrin and pyrethrum. In fumigation tests with female P. h. capitis at 0.25 mg/cm(2), 1,8-cineole, (-)-alpha-pinene, (E)-pinocarveol, and 1-alpha-terpineol were more effective in closed cups than in open ones, indicating that the effect of the monoterpenoids was largely due to action in the vapor phase. Neither delta-phenothrin nor pyrethrum exhibited fumigant toxicity. Eucalyptus leaf oil, particularly 1,8-cineole, 1-alpha-terpineol, and (E)-pinocaveol, merits further study as potential pediculicides or lead compounds for the control of P. h. capitis.},
	keywords = {animals, Oocytes/drug effects, Pediculus, Plant Extracts, Research Support, Non-U.S. Gov{\textquoteright}t, Terpenes/pharmacology},
	isbn = {0021-8561},
	author = {Yang,Y. C. and Choi,H. Y. and Choi,W. S. and Clark,J. Marshall and Ahn,Y. J.}
}
