@article {116,
	title = {Characterization of body louse midgut proteins recognized by resistant hosts},
	journal = {Medical and Veterinary Entomology},
	volume = {10},
	year = {1996},
	month = {1996},
	pages = {35 - 38},
	abstract = {The human body louse, Pediculus humanus, showed eighteen midgut proteins ranging between 12 and 117 kDa, when analysed by SDS- PAGE electrophoresis. Seven of them (12 kDa, 17 kDa, 29 kDa, 35 kDa, 40 kDa, 55 kDa and 97 kDa) were major bands based on their intensity of staining. The immunization of rabbits with a midgut extract elicited the production of protective polyclonal antibodies. These antibodies reacted strongly with all major midgut proteins as well as with 63 kDa and 117 kDa proteins when tested by the Western blot technique. The analysis of the proteins revealed that the 12 kDa, 25 kDa, 29 kDa, 35 kDa, 45 kDa, 87 kDa and 97 kDa proteins are glycosylated and none of them contained a lipid moiety. By electroelution, the proteins of 35 kda and 63 kDa were purified. On trypsinization, the proteins of 35 kDa and 63 kDa produced four major fragments (F- 1, F-2, F-3, and F-4) when resolved on a 18\% SDS-PAGE. The F-1 fragment of the 35 kDa protein reacted with the polyclonal antibodies by the immunoblot technique.},
	keywords = {adult, antigens, arthropod, body, Boophilus, cattle, control, human, immunization, immunogens, immunological, louse, midgut, pdf, Pediculus humanus, protein, ticks, vaccinated, vaccine},
	url = {http://www.phthiraptera.info/Publications/38618.pdf},
	author = {Ochanda,James O. and Mumcuoglu,Kosta Y. and Ben-Yakir,David and Okuru,J. K. and Oduol,V. O. and Galun,Rachel}
}
@article {119,
	title = {Localization of immunogenic antigens on midgut of the human body louse Pediculus humanus humanus (Anoplura: Pediculidae)},
	journal = {Journal of Medical Entomology},
	volume = {33},
	year = {1996},
	month = {1996},
	pages = {74 - 77},
	abstract = {Immunogenic midgut antigens of the human body louse, Pediculus humanus humanus L., were localized using rabbit antisera against a louse-midgut extract followed by a end antibody conjugated to either fluorescein or colloidal gold. Strong fluorescence was observed on the outer membrane of the epithelial cell of the midgut. The immunogold technique revealed that most of the antigens were localized on the microvilli of the midgut cells. Small numbers of gold particles were also seen in the gut lumen and within the cell cytoplasm. Only a few gold particles were seen in the lumen of the gut sections incubated with control sera.},
	keywords = {antigen, arthropod, Boophilus, immunization, immunofluorescence, labeling, localization, pdf, Pediculus humanus, ticks},
	url = {http://www.phthiraptera.info/Publications/38616.pdf},
	author = {Mumcuoglu,Kosta Y. and Rahamim,Ezra and Ben-Yakir,David and Ochanda,James O. and Galun,Rachel}
}
