Publication Type: | Journal Article |
Year of Publication: | 2005 |
Authors: | P. J. Waniek, Hendgen-Cotta, U. B., Stock, P., Mayer, C., Kollien, A. H., Schaub, G. A. |
Journal: | Parisitological Research |
Volume: | 97 |
Pagination: | 486 - 500 |
Date Published: | 2005 |
Abstract: | After the previous characterization of one trypsin gene (Try1) of the human body louse Pediculus humanus, genes encoding a second trypsin (Try2) and a chymotrypsin (Chy1) have been cloned using degenerate serine proteinase primers and 5¢- and 3¢-RACE, and sequenced. The deduced 259 and 267 amino acid sequences of Try2 and Chy1 show an identity of 33%–40% to trypsinogens and chymotrypsinogens of other insects. Considering previously published partial sequences, P. humanus possesses at least one Try1 gene, five variants/isoforms of Try2 and six variants/isoforms of Chy1. The genomic DNA of Try2 contains three introns and Chy1 contains five introns. Using wholemount in situ hybridization, gene expression of Try1, Try2 and Chy1 has been localized not only in the distensible |
URL: | http://www.phthiraptera.info/Publications/47183.pdf |